Roche applied science

国外一些知名的大公司的网站上都有很好的技术资料（Tech support），其实，这是一个非常好的资源，应该利用起来。像Sigma、Roche、GE、NEB、Millipore、Abcam等，登录它们的网站之后，能很容易找到这些资源，其中有关于试剂的使用说明注意事项、某个实验方法的流程和原理细节等。这些资源可能比我们手上的什么参考书更有价值。在Redondo日记本的右侧栏“我的链接”中收藏了几个著名生物公司的网址，朋友们可以参考。这篇日志里要推荐的是，Roche和Qiagen的网站上的类似《分子克隆》性质的实验指南！

如何设计引物？PCR应该注意什么？

如何选择限制性双酶切的buffer？

连接反应的温度和时间怎么控制？

应该制备多大浓度的核酸胶？多大电压跑电泳？

提取RNA时要注意哪些细节？

蛋白怎样鉴定？

等........

这些分子生物学的最基本的实验技能都能找到。

Qiagen Bench Guide 下载地址：

http://www1.qiagen.com/literature/BenchGuide/pdf/1017778_BenchGuide.pdf

Roche版分子克隆下载地址：

https://www.roche-applied-science.com/fst/publications.jsp?page=/PROD_INF/MANUALS/labfaqs/index.htm

另外推荐Roche的Technical Mannuals:（哎，到底是大公司啊！）

https://www.roche-applied-science.com/fst/publications.jsp?page=/PROD_INF/MANUALS/labfaqs/index.htm

其中包括：

• Apoptosis and Cell Proliferation, 3rd Edition
  This guide presents an overview of cell proliferation and cell death, along with the major assays currently available to measure each. In addition, the Guide clearly lists the advantages and disadvantages of each assay method - to help you determine which assay is best for your application.
   
  
• Biochemical Pathways Wallcharts
  Two informative wall charts, representing metabolic research in many life science fields, including molecular biology, cell biology, receptors and immunological interactions, and much more! The charts make it easy to find the metabolic pathways that most interest you. A digitized version of the pathways map is available at the ExPASy Molecular Biology Server.
   
  
• DIG Application Guide for Filter Hybridization
  This contains the definitive protocols and technical tips for using the DIG System in blotting techniques. The application procedures of the Guide are segmented into three major divisions: Labeling, Hybridization and Detection.
   
  
• DIG Product Selection Guide
  Decide which products are most suitable for your specific application and review a complete list of available products for nonradioactive labeling and detection.  
  
  
• Epitope Tagging: Basic Laboratory Methods
  This manual provides step-by-step details for simple protein characterization. The manual includes the actual procedures used to generate epitope-tagged proteins and subsequently how to detect them.
   
  
• Molecular Weight Markers for Nucleic Acids
  Precise sizing of nucleic acids has never been so easy: This brochure gives a detailed overview of all available DNA and RNA markers, unlabeled or DIG labeled.
   
  
• Nonradioactive In Situ Hybridization Application Manual
  This manual provides complete in situ hybridization protocols (e.g. RNA in situ hybridization, FISH) for using the DIG System on whole chromosomes, fixed sections, and cultured cells.
  
  
• New! Nonradioactive In Situ Hybridization Frequently Asked Questions
  Answers to frequently asked questions related to different aspects of in situ hybridization (e.g., General ISH, FISH, Probes, Fixatives, Hybridization, Detection, Troubleshooting).
   
  
• Nucleic Acid Isolation and Purification Manual, 2^nd Edition
  Consult this updated manual to see how Roche Applied Science’s nucleic acid preparation products can help you optimize your nucleic acid isolation results. Rely on the manual’s in-depth product descriptions to guide you in selecting the ideal product for your application.
   
  
• PCR Manual, 2^nd Edition
  This manual contains detailed protocols on performing PCR as well as preparation of templates and post-PCR clean-up. Important applications such as PRINS, cycle sequencing, detection of human chromosomes, and non-radioactive labeling are discussed.
  
  
• Restriction Enzymes Frequently Asked Questions and Ordering Guide 
  Roche Applied Science introduced the first restriction enzymes in 1976. Since then many researchers have chosen to apply our enzymes in their everyday work and have relied on the quality and consistency we provide. We have prepared Frequently Asked Questions which also include questions focused on troubleshooting.
   
  
• RTS Application Manual for Cell-Free Protein Expression
  See for yourself how cell-free protein expression with the Rapid Translation System (RTS) can be applied in a wide range of research projects. The 230-page RTS Application Manual provides a comprehensive overview of the RTS-technology and its applications.
   
  
• The Complete Guide for Protease Inhibition
  This brochure gives detailed technical information on the specificity, stability and starting concentration of common protease inhibitors.